Chellenge study, 1999.
I did not put all the tests in the experiment because I think we shall discuss it first. I do not know if I can perform all that I would like to.
GROUP 1 & 2 INTRANASAL AND INTRAVAGINAL INFECTION (7 HEIFERS, NEGATIVE ON BLV) | |||
Days |
Samples to be taken |
Tests |
Response |
-5 |
blood and urine |
mat all serovars + isolation from urine + ELISA BLV |
|
-4 |
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-3 |
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-2 |
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-1 |
blood |
mat |
FEEDBACK |
1 |
Intranasal inoculation - I will scratch the nasal plane with sharp fabric and then spray it with hardjo. I will also spray nasal cavity. The procedure will be repeated three days. Intravaginal inoculation of 10 ml of culture to anterior vagina with plastic pipette. |
||
2 |
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3 |
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4 |
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5 |
blood, urine, vag. mucus (VM), nasal mucus (NM), milk, saliva |
mat, wb-blood, milk, nasal mucus, saliva and vaginal mucus |
|
6 |
|||
7 |
blood, urine, vag. mucus (VM), nasal mucus (NM), milk, saliva |
mat, wb-blood, milk, nasal mucus, saliva and vaginal mucus |
|
8 |
|||
9 |
blood, urine, vag. mucus (VM), nasal mucus (NM), milk, saliva |
mat, wb-blood, milk, nasal mucus, saliva and vaginal mucus |
|
10 |
|||
11 |
|||
12 |
|||
13 |
blood, urine, vag. mucus (VM), nasal mucus (NM), milk, saliva |
mat, wb-blood, milk, nasal mucus, saliva and vaginal mucus |
|
14 |
|||
15 |
|||
16 |
|||
17 |
blood, urine, vag. mucus (VM), nasal mucus (NM), milk, saliva |
mat, wb-blood, milk, nasal mucus, saliva and vaginal mucus |
|
18 |
|||
19 |
|||
20 |
|||
21 |
blood, urine, vag. mucus (VM), nasal mucus (NM), milk, saliva |
mat, wb-blood, milk, nasal mucus, saliva and vaginal mucus |
|
22 |
|||
23 |
|||
24 |
|||
25 |
blood, urine, vag. mucus (VM), nasal mucus (NM), milk, saliva |
mat, wb-blood, milk, nasal mucus, saliva and vaginal mucus |
|
26 |
|||
27 |
|||
28 |
|||
29 |
blood, urine, vag. mucus (VM), nasal mucus (NM), milk, saliva |
mat, wb-blood, milk, nasal mucus, saliva and vaginal mucus |
|
30 |
|||
31 |
|||
32 |
|||
33 |
|||
34 |
|||
35 |
blood, urine, vag. mucus (VM), nasal mucus (NM), milk, saliva |
mat, wb-blood, milk, nasal mucus, saliva and vaginal mucus |
|
36 |
|||
37 |
|||
38 |
|||
39 |
|||
40 |
|||
41 |
|||
42 |
blood, urine, vag. mucus (VM), nasal mucus (NM), milk, saliva |
mat, wb-blood, milk, nasal mucus, saliva and vaginal mucus |
|
43 |
|||
44 |
|||
45 |
|||
46 |
|||
47 |
|||
48 |
|||
49 |
blood, urine, vag. mucus (VM), nasal mucus (NM), milk, saliva |
mat, wb-blood, milk, nasal mucus, saliva and vaginal mucus |
|
50 |
|||
51 |
|||
52 |
|||
53 |
|||
54 |
|||
55 |
|||
56 |
blood, urine, vag. mucus (VM), nasal mucus (NM), milk, saliva |
mat, wb-blood, milk, nasal mucus, saliva and vaginal mucus |
I did not put all the tests in the experiment because I think we shall discuss it first. I do not know if I can perform all that I would like to.