Sampling methods
Blood
Method of sampling | Storage of samples |
Sterile from jugular vein by the use of vaccujet (Terumo vacum tubes 10 ml
with clothing factor) Every time I will take two tubes. |
Storage in polypropilene tubes and deepwell plate -20 C |
Urine
Method of sampling | Storage of samples |
Administration of 500 mg of Lasix. Vulva will be cleaned with normal saline and then I will collect urine in 50 ml centrifuge tubes. Then I will transfer 250 uL to culture tube immidiatelly (on the site) with sterile pipette tips for isolation. | I need procedure for treatment and
storage of urine for PCR.
|
Vaginal mucus, saliva and nasal mucus
Method of sampling | Storage of samples |
I will take mucus using sponge. I will leave sponges 3-5 minutes in vagina, mouth and nose and then I will pull them out. After that I will put sponges in 20 ml sterile plastic syringes and squize contents out. | Storage for serology in polypropylene tubes + deepwell plates
without pretreatment. I need procedure for treatment and storage of mucus for PCR. |