Purpose of study

Investigation Of Immunological Response Of Heifers To Leptospira interrogans Serovar hardjo After Experimental Intranasal And Intravaginal Infection BASIC QUESTION: HOW DOES THE IMMUNE RESPONSE LOOK LIKE AND CAN WE DEVELOP SIMPLE TEST FOR ANTIBODY DETECTION IN MILK AND SERUM Includes: Timetable of immune response (time of appearance of IgM, IgG, IgA, and presence of these in saliva, milk, nasal mucus and vaginal mucus) Investigation of hardjo antigens involved in induction of immune response during accute infection. Development of ELISA and cELISA for detection of hardjo antibodies in milk and serum |
Comparative study of different methods for detection of Leptospira interrogans serovar hardjo antigen in urine, milk and vaginal mucus of experimentally infected heifers BASIC QUESTION: DOES IT SHED LEPTO IN URINE, VAGINAL MUCUS OR MILK Includes: Comparative study of MIPA-PCR, Garritsen PCR, immunoflorescence (with Hans Korver monoclonals) for detection of lepto in urine, milk and vaginal mucus (culture as reference for urine) |