Leptospira Laboratory Page
Author: Miladin Kostovic

There has been a general desire for many years for the utopian concept of a cheap, reliable, sensitive, specific serological test which could be performed in the most simply equiped laboratories, and many possibilities have been investigated, but in the real world, things are never quite what one hopped for.

Laboratory: Minimum Requirements For Establishing a Small Leptospira Laboratory Microscope Culture: Media Purification of contaminated cultures Direct isolation Indirect isolation Serology: MAT ELISA Slide agglutination Rapid Latex Agglutination Assay (Lepto RLA) IgM DipStick Indirect Immunofluorescence Test (IIFT) GENERAL PROTOCOLS Preparation Of Hyperimmune Sera To Leptospires In Rabbits The MacFarland Nephelometric Method to Estimate Numbers of Bacteria Relationship Between optical Density And Number of Bacteria

THE PROBLEMS OF DIAGNOSIS ARE SUCH THAT DIAGNOSIS IS BEST ESTABLISHED BY A COMBINATION OF AS MENY PARAMETERS AS POSSIBLE, HAVING DUE REGARD TO THE VALUE OF EACH FEATURE AT THE VARIOUS STAGES OF INFECTION.

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