Chellenge study, 1999.

 

I did not put all the tests in the experiment because I think we shall discuss it first. I do not know if I can perform all that I would like to.

GROUP 1 & 2 INTRANASAL AND INTRAVAGINAL INFECTION (7  HEIFERS, NEGATIVE ON BLV)

Days

Samples to be taken

Tests

Response

-5

blood and urine

mat all serovars + isolation from urine + ELISA BLV

FEEDBACK

-4

     

-3

     

-2

-1

blood

mat

FEEDBACK

1

Intranasal inoculation - I will scratch the nasal plane with sharp fabric and then spray it with hardjo. I will also spray nasal cavity. The procedure will be repeated three days.

Intravaginal inoculation of 10 ml of culture to anterior vagina with plastic pipette.

2

3

4

5

blood, urine, vag. mucus (VM), nasal mucus (NM), milk, saliva

mat, wb-blood, milk, nasal mucus, saliva and vaginal mucus

FEEDBACK

6

7

blood, urine, vag. mucus (VM), nasal mucus (NM), milk, saliva

mat, wb-blood, milk, nasal mucus, saliva and vaginal mucus

FEEDBACK

8

9

blood, urine, vag. mucus (VM), nasal mucus (NM), milk, saliva

mat, wb-blood, milk, nasal mucus, saliva and vaginal mucus

FEEDBACK

10

11

12

13

blood, urine, vag. mucus (VM), nasal mucus (NM), milk, saliva

mat, wb-blood, milk, nasal mucus, saliva and vaginal mucus

FEEDBACK

14

15

16

17

blood, urine, vag. mucus (VM), nasal mucus (NM), milk, saliva

mat, wb-blood, milk, nasal mucus, saliva and vaginal mucus

FEEDBACK

18

19

20

21

blood, urine, vag. mucus (VM), nasal mucus (NM), milk, saliva

mat, wb-blood, milk, nasal mucus, saliva and vaginal mucus

FEEDBACK

22

23

24

25

blood, urine, vag. mucus (VM), nasal mucus (NM), milk, saliva

mat, wb-blood, milk, nasal mucus, saliva and vaginal mucus

FEEDBACK

26

27

28

29

blood, urine, vag. mucus (VM), nasal mucus (NM), milk, saliva

mat, wb-blood, milk, nasal mucus, saliva and vaginal mucus

FEEDBACK

30

31

32

33

34

35

blood, urine, vag. mucus (VM), nasal mucus (NM), milk, saliva

mat, wb-blood, milk, nasal mucus, saliva and vaginal mucus

FEEDBACK

36

37

38

39

40

41

42

blood, urine, vag. mucus (VM), nasal mucus (NM), milk, saliva

mat, wb-blood, milk, nasal mucus, saliva and vaginal mucus

FEEDBACK

43

44

45

46

47

48

49

blood, urine, vag. mucus (VM), nasal mucus (NM), milk, saliva

mat, wb-blood, milk, nasal mucus, saliva and vaginal mucus

FEEDBACK

50

51

52

53

54

55

56

blood, urine, vag. mucus (VM), nasal mucus (NM), milk, saliva

mat, wb-blood, milk, nasal mucus, saliva and vaginal mucus

FEEDBACK

I did not put all the tests in the experiment because I think we shall discuss it first. I do not know if I can perform all that I would like to.