Sampling methods

Blood

Method of sampling | Storage of samples
Sterile from jugular vein by the use of vaccujet (Terumo vacum tubes 10 ml with clothing factor) Every time I will take two tubes. | Storage in polypropilene tubes and deepwell plate -20 C

Urine

Method of sampling | Storage of samples
Administration of 500 mg of Lasix. Vulva will be cleaned with normal saline and then I will collect urine in 50 ml centrifuge tubes. Then I will transfer 250 uL to culture tube immidiatelly (on the site) with sterile pipette tips for isolation. | I need procedure for treatment and storage of urine for PCR.

Vaginal mucus, saliva and nasal mucus

Method of sampling | Storage of samples
I will take mucus using sponge. I will leave sponges 3-5 minutes in vagina, mouth and nose and then I will pull them out. After that I will put sponges in 20 ml sterile plastic syringes and squize contents out. | Storage for serology in polypropylene tubes + deepwell plates without pretreatment. I need procedure for treatment and storage of mucus for PCR.